Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Alterations and Severe Abnormalities in Spermatozoa of Young Men Living in the “Valley of Sacco River” (Latium, Italy): A Preliminary Study

doi: 10.3390/ijerph191711023

Figure Lengend Snippet: DNA-binding ability of SNBPs obtained from control group ( a ) and VSR group ( b , c ) analyzed by electrophoretic mobility shift assay (EMSA) on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a ratio w/w with increasing amount of SNBPs from samples. VSR: Valley of Sacco River; control: Valley of Sele River.

Article Snippet: pGEM3 plasmid (2867 bp) from Escherichia coli HB 101 cells was prepared by using the HiPura Endotoxin free Plasmid DNA Midiprep Kit (HiMedia Laboratories Pvt.

Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Agarose Gel Electrophoresis, Plasmid Preparation, Incubation

Journal: Cytotechnology

Article Title: Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

doi: 10.1007/s10616-020-00433-4

Figure Lengend Snippet: Agarose gel electrophoresis of SC, OC and linear forms of four plasmids. Lane 1, native plasmid DNA of VSVG; lane 2, 7-day treatment at 50 °C for plasmid DNA of VSVG; lane 3, a mixture of native VSVG plasmid DNA and heat-treated VSVG plasmid DNA at ratio of 1:1; lane 4, native plasmid DNA of MDK; lane 5, 7-day treatment at 50 °C for plasmid DNA of MDK; lane 6, a mixture of native MDK plasmid DNA and heat-treated MDK plasmid DNA at ratio of 1:1; lane 7, native plasmid DNA of RSK; lane 8, 7-day treatment at 50 °C for plasmid DNA of RSK; lane 9, a mixture of native RSK plasmid DNA and heat-treated RSK plasmid DNA at ratio of 1:1; lane 10, native plasmid DNA of pLenti6.3/V5-CD19 CAR; lane 11, 7-day treatment at 50 °C for plasmid DNA of pLenti6.3/V5-CD19 CAR; lane 12, a mixture of native pLenti6.3/V5-CD19 CAR plasmid DNA and heat-treated pLenti6.3/V5-CD19 CAR plasmid DNA at ratio of 1:1

Article Snippet: The next day, the plasmid DNA was extracted using the AxyPrep Plasmid Midiprep Kit (Axygen, Corning Incorporated, USA).

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

Journal: Cytotechnology

Article Title: Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

doi: 10.1007/s10616-020-00433-4

Figure Lengend Snippet: Effect of the SC plasmid DNA proportion on the functional titer of lentiviral vectors produced by HEK293T cells. Functional titer was measured by transduction units (TU). Lentivirus samples in three groups were harvested individually. Error bars represent the standard deviation of three independent infections. *P < 0.05; **P < 0.01

Article Snippet: The next day, the plasmid DNA was extracted using the AxyPrep Plasmid Midiprep Kit (Axygen, Corning Incorporated, USA).

Techniques: Plasmid Preparation, Functional Assay, Produced, Transduction, Standard Deviation

Journal: Cytotechnology

Article Title: Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

doi: 10.1007/s10616-020-00433-4

Figure Lengend Snippet: Effect of the SC plasmid DNA proportion on the functional titer of lentiviral vectors produced by HEK293SF cells. Functional titer was measured by transduction units (TU). Lentivirus samples in three groups were harvested individually. Error bars represent the standard deviation of three independent experiments. *P < 0.05; **P < 0.01; NS, no significant difference

Article Snippet: The next day, the plasmid DNA was extracted using the AxyPrep Plasmid Midiprep Kit (Axygen, Corning Incorporated, USA).

Techniques: Plasmid Preparation, Functional Assay, Produced, Transduction, Standard Deviation

Journal: Cytotechnology

Article Title: Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

doi: 10.1007/s10616-020-00433-4

Figure Lengend Snippet: Agarose gel electrophoresis of SC, OC and linear forms of four plasmids after heat or enzyme treatment. Lane 1, native plasmid DNA of RSK; lane 2, 7-day treatment at 50 °C for plasmid DNA of RSK; lane 3, 10-day treatment at 50 °C for plasmid DNA of RSK; lane 4, RSK plasmid DNA was digested with Xho I; lane 5, native plasmid DNA of VSVG; lane 6, 7-day treatment at 50 °C for plasmid DNA of VSVG; lane 7, 10-day treatment at 50 °C for plasmid DNA of VSVG; lane 8, VSVG plasmid DNA was digested with Hand III; lane 9, native plasmid DNA of MDK; lane 10, 7-day treatment at 50 °C for plasmid DNA of MDK; lane 11, 10-day treatment at 50 °C for plasmid DNA of MDK; lane 12, MDK plasmid DNA was digested with Mun I; lane 13, native plasmid DNA of pLenti6.3/V5-CD19 CAR; lane 14, 7-day treatment at 50 °C for plasmid DNA of pLenti6.3/V5-CD19 CAR; lane 15, 10-day treatment at 50 °C for plasmid DNA of pLenti6.3/V5-CD19 CAR; lane 16, pLenti6.3/V5-CD19 CAR plasmid DNA digested with Sal I

Article Snippet: The next day, the plasmid DNA was extracted using the AxyPrep Plasmid Midiprep Kit (Axygen, Corning Incorporated, USA).

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

Journal: iScience

Article Title: DNA methyltransferases are complementary in maintaining DNA methylation in embryonic stem cells

doi: 10.1016/j.isci.2022.105003

Figure Lengend Snippet:

Article Snippet: AxyPrep Endo-Free Plasmid Midiprep Kit , Axygen of Corning , Cat. # 09318KA2.

Techniques: Virus, Recombinant, Gel Extraction, Plasmid Preparation, Methylation, Software